FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372653
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGGGGAAACATGTTGTCTTCCTAAGACCGCTTGGCC51300.3737288302287614No Hit
GTGAGCCAAGGAGTTGACCAGGAAGGTTGTCTTCCTAAGACCGCTTGGCC18070.1316428842540686No Hit
GTGAGCCAAGGAGTTGAATCTATCAATTGTCTTCCTAAGACCGCTTGGCC15580.11350282992132754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC3700.083.99942
TGAGCGA8600.078.301762
CGAAGGA6900.078.2960056
CTTGGGT112950.078.111433
AGTAGGG31600.078.086892
TCTTGGG81350.078.030842
ACCGACC3950.077.8881153
GAGTAGG31450.077.226991
TAGGGTG74700.076.954574
CTCTTGG44250.076.758111
GTAGGGT55800.076.596423
AAACCGA6750.075.8615951
ATTGGGT50000.074.68993
AACGAGT8000.074.5699168
AGCGAAG8150.073.7267154
CATGGAG26200.073.6331257
GACAAGG23950.073.5204855
AGGGTGT77650.073.515145
GTGAGGC30200.072.9566961
GTCTTGG61450.072.627481
Produced by FastQC (version 0.11.5)