FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676493
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACATGTTTCCCCAACTCCTT129420.3520202540845311No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAACATGTTTCCCCAACTCCTT87930.23916814203100617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG376300.086.460972
GTGTAGG405250.086.2843551
GTAGGGT663450.083.544713
GAGTAGG293600.083.093941
AGTAGGG278850.082.639582
TAGGGTG702100.082.198184
TCGGACG1350.076.651973
AGGGTGT801450.076.033535
GTAGGCT60950.075.937413
TGTTGCG40650.074.633453
GTTGCGT51700.073.690424
AGTTGCG11450.072.711343
GAGTTGG364200.072.158851
ACGGAGG19000.071.792453
GTTGGCT211350.071.328023
AGTTGGG342050.070.668842
GGCTGTG308750.070.503366
GTCGGGT11200.070.134683
GCTGTGT317950.069.373837
GATGCGT16950.069.094944
Produced by FastQC (version 0.11.5)