FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676493
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGGGGAAACATGTTGTCTTCCTAAGACCGCTTGGCC129400.35196585441615147No Hit
GTGAGCCAAGGAGTTGACCAGGAAGGTTGTCTTCCTAAGACCGCTTGGCC62070.1688293708161555No Hit
GTGAGCCAAGGAGTTGAATCTATCAATTGTCTTCCTAAGACCGCTTGGCC51820.14094954077159946No Hit
GTGAGACAAGGAGTTGGGGAAACATGTTGTCTTCCTAAGACCGCTTGGCC39260.10678654902919711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT344150.088.445663
TAGGGTG449700.088.4436344
TGTAGGG293900.086.5228652
GTGTAGG287800.085.792781
AGGGTGT467100.084.5743945
ATAGGGT69300.080.92143
ACCGACC9600.080.351623
CTAGGGT70300.079.972273
TATAGGG51200.079.833742
TCTTGGG211900.079.0477752
AACCGAC9250.077.890852
TCTAGGG62650.077.7206042
CTCTTGG118450.077.063421
GTATAGG50800.076.797751
AACGAGT20550.076.475828
TGAGCGA21300.075.831782
AGTAGGG69700.075.1967542
GTGAGGC92950.074.235351
TATTGGG69750.073.995122
GTCTAGG51450.073.634911
Produced by FastQC (version 0.11.5)