FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605835
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAAGGTAGAGAGTCAACTCCTT415242.5858198382772826No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT374122.329753679549891No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT367532.2887158394231037No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT345052.1487263635429543No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT311641.9406726095769493No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT253561.578991614954214No Hit
GTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC165441.030242833167791No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT149880.9333462030656948No Hit
AGTCGGAGGCCAAGCGCTCTTAGGAAGACAAGGTAGAGAGTCAACTCCTT87640.5457596826573091No Hit
GTGAGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC83400.5193559736834731No Hit
GTGGGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT70330.437965295313653No Hit
CTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT58450.3639850918680936No Hit
CTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT58200.3624282694050136No Hit
CTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT55400.3449918578185181No Hit
CTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT52910.32948590608624173No Hit
GTGGGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT50120.31211176739826946No Hit
CTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT45510.28340396117907507No Hit
ATGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT42380.263912543941314No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATGACTAGACCCAACTCCTT41420.257934345683087No Hit
ATGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT38220.23800701815566358No Hit
GTGAGCCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC37260.23202881989743654No Hit
ATGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT36200.22542789265397753No Hit
CTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC32630.20319646788119577No Hit
GTGAGGCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC32410.20182646411368543No Hit
GTGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGC31860.1984014546949095No Hit
CTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT31800.19802781730377034No Hit
GTGAGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGC31530.19634644904364396No Hit
GTGAGCCAACGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC28870.17978185803647326No Hit
GTGAGGCAACGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC28180.17548502803837257No Hit
GTGAGCCAAGCGCTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT28100.17498684485018698No Hit
ATGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT27330.1701918316639007No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTT26240.1634040857248721No Hit
GTGAGCCAAGCGCTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT22730.14154629834322954No Hit
GTGGGCCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC22610.14079902356095114No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAAGGTAGAGAGTCAACTCCTT20590.12821989805926512No Hit
GTGAGCCAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC20300.1264139840020924No Hit
ATGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT20290.1263517111035692No Hit
GCGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT19970.12435897835082682No Hit
GCGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT19490.1213698792217133No Hit
CTGAGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC18730.11663713893395025No Hit
GTGAGGCAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC17950.11177985284914078No Hit
GTGGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC17950.11177985284914078No Hit
TCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTCACAAGTCGGAGG17650.10991166589344485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA409.094947E-1294.1709063
GTGAGGC117950.091.651071
GTGAGCC109750.090.204631
TGAGCCA143400.090.011272
GTGGGCC63750.088.26351
TGGGCCA82900.088.1337362
TGAGGCA138750.087.7859042
ACGCAAG9250.087.028134
TCGGACG6550.086.263433
GACGCAA8800.084.5397953
CGCAAGC10650.084.391535
TGAGCAA49700.084.099472
AGTTGCG4150.083.95963
CTCGCGC452.7284841E-1183.84911
CGGACGC6800.083.7686844
TGTTGCG42650.083.682943
TGACGCA8800.083.1872
ACGGAGG9650.082.948463
GTGAGCA38700.082.752231
CGCAACG1950.082.046395
Produced by FastQC (version 0.11.5)