FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605835
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC19450.094.851573
AACCGAT17000.091.455932
TGAGCGA82500.089.501372
GTGAGCG74700.088.62341
ACCGGTC6350.088.08613
CGAAGGA59550.087.7601246
GAGCGAA73650.087.405213
AACGACT17600.087.233298
ACCGAGC8000.087.091263
AAACCGA28650.087.048221
AACGAGT66800.086.802928
GCCAACG58300.086.286235
ACGAGTT67400.086.2189259
CCAACGA63100.085.916036
AACCGGT5600.085.0952762
CAACGAG64900.084.216727
GCGAACG10900.083.95225
TCAGCCA75900.083.3320162
CAACGAC17800.083.083627
AGCGAAG75750.082.827634
Produced by FastQC (version 0.11.5)