FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6735861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATGACTAGACCCAACTCCTT1917712.847015400109949No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT1337471.9855961992089801No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT1324781.9667567368150858No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT1267621.8818975035262753No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT1114751.6549480459884789No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT951581.412707299037198No Hit
GTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC656840.9751388872187237No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT588800.8741273016174176No Hit
AGTCGGAGGCCAAGCGCTCTTAGGAAGACAATGACTAGACCCAACTCCTT441770.6558478567179459No Hit
GTGAGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC344540.511501053837067No Hit
GTGGGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT276960.41117238019014946No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTT235850.3501408357446806No Hit
CTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT219080.3252442412336003No Hit
CTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT208810.309997489556272No Hit
GTGGGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT207500.3080526750774697No Hit
CTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT205970.305781250533525No Hit
CTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT202480.3006000272274027No Hit
CTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT187770.2787616905990192No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAATGACTAGACCCAACTCCTT179520.26651381315618No Hit
ATGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT173440.25748749862860887No Hit
ATGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT164260.24385895136494057No Hit
ATGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT149570.2220503065606609No Hit
CTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC139400.2069520140038519No Hit
GTGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGC136420.20252793221237791No Hit
GTGAGCCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC130190.19327892900402785No Hit
GTGAGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGC127550.18935960822231931No Hit
GTGAGGCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC126820.1882758566425287No Hit
CTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT116780.17337056094239472No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTT114110.16940670242453043No Hit
ATGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT113130.16795180304344165No Hit
GTGAGGCAACGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC112740.16737281247341654No Hit
GTGAGCCAACGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC108670.16133052626828256No Hit
TCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTCACAAGTCGGAGG100950.1498694821641955No Hit
GTGAGCCAAGCGCTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT99360.1475089821479392No Hit
GTGGGCCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC88860.13192077449341666No Hit
ATGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT86410.12828352604069473No Hit
GCGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT82760.12286476814174165No Hit
GCGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT82620.12265692537301467No Hit
CTGAGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC79590.11815861402128103No Hit
GTGGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC78080.11591688130144016No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAAGGTAGAGAGTCAACTCCTT77140.11452136556855909No Hit
GTGAGCCAAGCGCTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT74320.11033481836991588No Hit
GTGAGCCAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC72410.10749924916799798No Hit
GTGAGGCAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC68900.10228833403777186No Hit
AGTCGGACGCCAAGCGGTCTTAGGAAGACAATGACTAGACCCAACTCCTT68160.10118973654592932No Hit
ATGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT67610.10037321138307337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGC435450.090.251461
TGAGCCA531950.088.3393552
GTGAGCC402200.088.322311
GTGGGCC240700.087.327471
TGGGCCA317950.087.1963352
TGAGGCA521900.085.5714652
TCGGACG26850.085.390683
CGGACGC28500.085.066594
TGAGCAA196850.084.4503862
ACGGAGG59150.084.129073
GGGCCAA326450.083.471393
GACGCAA31650.083.4478153
TGTTGCG165800.083.055273
ACGCAAG34350.082.913514
AGTTGCG15800.082.5369643
GTGACGC20700.082.228761
AGTCCGA24300.081.8828661
GAGCCAA580400.081.511943
TGACGCA32550.081.083382
AGCAAGC198500.080.6808554
Produced by FastQC (version 0.11.5)