FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221603
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTT1439112.7560693526489852No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT1181962.2635960642737487No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT1167422.2357502092748147No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT1055922.0222142510642804No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT1036461.9849460022142624No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT831141.5917334197946493No Hit
GTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC598221.1456635060152982No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT494340.9467207675497352No Hit
AGTCGGAGGCCAAGCGCTCTTAGGAAGACAACACTTAGCTACAACTCCTT386210.739638766103053No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATGACTAGACCCAACTCCTT342710.6563310155904231No Hit
GTGAGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC292710.5605749805184347No Hit
GTGGGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT240360.46031841179806277No Hit
CTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT195130.37369750247194206No Hit
CTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT183810.35201833613164385No Hit
CTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT167750.3212614976665212No Hit
CTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT166750.3193463769650814No Hit
GTGGGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT160340.30707045326885246No Hit
ATGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT153780.2945072614674076No Hit
ATGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT153370.2937220619798173No Hit
CTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT151830.29077277609960006No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTT144400.2765434292879026No Hit
ATGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT125710.2407498233779933No Hit
CTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC121060.2318445121162984No Hit
GTGAGCCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC119120.22812917795550525No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTT117380.22479686793500003No Hit
GTGAGGCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC117290.22462450707187048No Hit
GTGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGC115160.22054529997780373No Hit
GTGAGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGC112260.21499144994362843No Hit
ATGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT102970.19719997862725297No Hit
GTGAGCCAACGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC100010.19153122135099127No Hit
GTGAGGCAACGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC99030.18965440306358028No Hit
CTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT98260.18817976012347168No Hit
AGTCGGAGGCCAAGCGCTCTTAGGAAGACAATGACTAGACCCAACTCCTT91870.17594213884127152No Hit
AGTCGGACGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTT89030.1705031960491826No Hit
GTGAGCCAAGCGCTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT84910.16261289875925075No Hit
GTGGGCCAACGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC80370.15391825077471422No Hit
GCGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT74660.1429829115694931No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAAGGTAGAGAGTCAACTCCTT74320.1423317705310036No Hit
GCGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT73660.14106779086805335No Hit
GTGAGCCAAGCGCTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT70300.13463298531121573No Hit
AGTCGGACGCCAAGCGCTCTTAGGAAGACAACACTTAGCTACAACTCCTT69810.13369457616751027No Hit
GTGGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC69340.13279446943783357No Hit
ATGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT68910.13197096753621446No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAATGACTAGACCCAACTCCTT66300.12697250250545664No Hit
GTGAGCCAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC65030.12454029921462814No Hit
CTGAGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTC64830.1241572750743402No Hit
GTGAGGCAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC64430.1233912267937643No Hit
GTGGACCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT60640.11613291933530757No Hit
ATGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT58460.11195795620616887No Hit
TCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCTCACAAGTCGGAGG55090.10550399944231686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGC377250.091.236491
TCGGACG32300.089.9362263
CGGACGC33800.089.699044
TGAGCCA458000.089.290552
GTGAGCC344550.089.207971
TGGGCCA276200.088.537072
GTGGGCC211100.088.5137561
ACGGAGG47100.088.065753
TGAGGCA444150.087.429182
ACGCAAG32050.087.252324
GACGCAA28800.086.8068543
GTGACGC21000.086.664841
TGAGCAA175000.086.259612
TGACGCA30250.086.162172
GGACGCC34750.085.173715
AGTTGCG11200.084.9151843
GGGCCAA284250.084.573123
TGTTGCG120550.084.165113
GTGAGCA146900.083.482121
CGCAAGC39100.082.076235
Produced by FastQC (version 0.11.5)