FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016485
Sequences flagged as poor quality0
Sequence length100
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG18200.086.6949845
ACCGATC16050.086.228563
TGAGCGA146800.084.9545442
AACCGAT15400.084.2096562
GTGAGCG135350.084.150441
CGAAGGA132950.083.4293756
GAGCGAA153350.082.6969153
AACGAGT107750.081.384558
CAACGAC24650.081.24587
TCAGCCA139550.081.126622
CGAAGCA34050.080.981666
CAGCGAA25800.080.463293
ACGAGTT108150.080.187239
AGCGAAG166800.079.3951344
ACAGATC57500.079.310753
AGCGAAC20350.078.4312064
TCAGCGA26150.078.369712
GTCAGCC74300.077.978461
GCGAAGG136450.077.676255
AACGACT26850.077.667958
Produced by FastQC (version 0.11.5)