FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813040
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT50730.27980629219432557No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT38400.21179896748003352No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT35710.1969620085602083No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT31030.1711490093985792No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTT29920.16502669549485946No Hit
GTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTG26800.14781802938710675No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT21600.11913691920751887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG120000.087.82193
GAGTTGG86500.085.476052
GTTGCGT137300.084.827714
AGTTGCG14000.084.4247363
TGAGTTG103900.084.3478851
AGTTGGG80700.084.142563
GTGTTGC133000.082.606382
GCGTGTC11250.080.3972557
TTGCGTG146500.079.53855
TGCGTGT147800.078.816686
GCGTGTG140050.076.690977
CGTGTCT11850.076.4515468
CGTGTGT144800.074.198978
GAGTTGC16450.072.040492
GAGCCAA28800.069.3261953
TCGGACG1500.069.064583
GGCAAGC25550.068.547535
GTGAGGC19900.068.18931
TGTGTTG982200.067.945831
AGCCAAG29650.066.712744
Produced by FastQC (version 0.11.5)