FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320533
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT70900.30553325464451486No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT52680.22701681036210214No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT49500.21331306212839896No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT43120.18581937856518307No Hit
GTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTG36590.15767929178339632No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTT32260.13901978554064948No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT29410.12673812438780227No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT23880.10290739239648822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG17350.088.2418753
TGTTGCG152350.088.092893
GAGTTGG113200.085.495822
GTTGCGT176200.084.540664
TGAGTTG137650.083.53241
GTGTTGC167850.082.98562
AGTTGGG109450.082.981663
TTGCGTG187250.079.418435
TGCGTGT189200.078.5960546
GCGTGTG181800.076.87767
GCGTGTC12050.075.473217
CGTGTGT188550.074.144328
GAGCCAA39400.071.8573
GTGAGGC25650.070.731811
CGTGTCT12850.070.5208368
AGCCAAG40300.069.553924
GAGTTGC22300.068.626132
GGCAAGC35550.067.953025
GTTCGGT15050.067.790152
TGTGTTG1267000.066.9405061
Produced by FastQC (version 0.11.5)