FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13065165
Sequences flagged as poor quality0
Sequence length100
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGT464700.076.711383
TCTTGGG465350.075.3198552
TGAGCGA117000.070.406662
GTGAGCG106150.070.0275651
CTCTTGG292000.069.0535661
ATTGGGT233250.068.912493
CGTGTTG143500.067.084771
GAGCGAA101050.066.63923
AGCGAAG105200.065.766964
TATTGGG191400.064.0614242
CGAAGGA87750.063.0127036
AACGAGT69400.062.6678928
GCGAAGG85200.062.3774075
CTGTTTG435550.062.2798355
GTCTTGG331200.060.6527021
ACGAGTT73150.060.561329
TGTGTTG1581650.060.548591
TGCTCGG51600.058.8666081
TCTGTTG390900.058.5206151
CAACGAG69400.057.7896047
Produced by FastQC (version 0.11.5)