FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885038
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG32150.076.627353
GTGTAGG177350.076.2274861
TGTAGGG163500.075.522722
GAGTAGG139400.069.425071
AGTAGGG125600.068.459152
TGAGTTG93800.065.779971
AGTTGCG6300.065.6712953
TGATGCG8600.063.415153
GAAGGGT194200.061.3198621
AAGGGTG195250.060.907552
TTCGGTC4750.060.3767433
TGTGATG122450.059.094221
GAGAGTT66850.058.688782
GCGTGTC7700.058.5809147
GTTCGGT9350.057.790472
CTTGCGT7950.056.70291
GTTGCGT50000.056.599834
GATGCGT14550.056.2179344
GGAGAGT71150.054.711771
TTGCGTC8000.054.0340962
Produced by FastQC (version 0.11.5)