FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570656
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG451500.077.701861
TGTTGCG69850.077.668593
TGTAGGG412750.077.458242
GAGTAGG347600.073.088671
AGTAGGG321150.072.56052
TGAGTTG213350.066.769051
AGTTGCG14050.063.574553
TGATGCG17500.059.637713
GAAGGGT426250.059.36031
TTGCGTC15600.058.726832
TGTGATG270450.058.574741
AAGGGTG435000.058.0194052
GAGTTGG519750.057.968211
AGTTGGG473500.057.3203662
GTTCGGT21650.057.2891732
GTTGCGT109650.056.6762244
GAGAGTT147650.056.225022
GTAGGGT984400.055.851483
TAGGGTG1026450.055.5521774
GATGCGT30400.055.513544
Produced by FastQC (version 0.11.5)