FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570656
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGT45700.081.0586851
AGTTGGG720400.080.691022
GAGTTGG703900.080.128581
ATGCGTG61600.078.065372
GATGGGT495850.077.276981
ATGGGTG553450.076.3356552
GATCGGT22900.070.617531
AGTTAGG95550.066.200112
ATCGGTG29350.065.95492
GACTTGG121850.065.431951
GAGTTAG99450.063.3889471
ATGAGTG133300.063.28182
AGTAGGG105550.062.9625242
GAGTAGG107500.062.271751
AATTGGG87550.061.3284572
CAGTTGG93700.060.8570331
GAATTGG81750.060.437221
CATGCGT25800.060.311181
TGTTGCG56100.059.703193
ATCGGTC7200.059.5281332
Produced by FastQC (version 0.11.5)