FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364108
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTT110790.3293294983395301No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTT81340.24178771906252713No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT80800.24018253872943435No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT68310.20305531213623343No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT63030.18736021554599316No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT62200.18489299392290615No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT56840.16896009283887437No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT50680.1506491468169274No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT49940.14844945524935585No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT45020.1338244788811774No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT38020.1130165856744195No Hit
GAGAGGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT35710.10614998091618938No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT33990.1010371842996717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG20750.087.0754853
TGTTGCG100200.086.0281453
GTTGCGT124350.083.352594
GATGCGT35650.082.748174
TGATGCG21350.081.542993
AGATGCG15400.081.2725143
CGGACGC2850.079.240414
TTGCGTG132000.078.451815
ATGCGTG38050.077.653655
TGAGTTG146600.077.5550461
TGCGTGT173450.076.684776
GCGTGTC15500.075.1927
GTGTAGG177500.074.5089951
ACGGAGG18500.074.266663
GTGTTGC119550.074.183832
TGTAGGG165100.073.796792
TCGGACG1950.072.388473
GCGTGTG186750.071.140487
GAGCCAA80750.070.9720843
TCCGACG200.002153783670.5787663
Produced by FastQC (version 0.11.5)