FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364108
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGGCC119720.35587442495900845No Hit
GTGAGCCAAGGAGTTGTCAGTGAGTCTTGTCTTCCTAAGACCGCTTGGCC68460.20350119556209254No Hit
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGGCT63330.18825198239771138No Hit
GTGAGCCAAGGAGTTGACTGCCTTATTTGTCTTCCTAAGACCGCTTGGCC57260.17020856643127985No Hit
GTGAGCCAAGGAGTTGCCTTAATTAATTGTCTTCCTAAGACCGCTTGGCC51520.15314609400173834No Hit
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGCCT48500.14416897436110848No Hit
GTGAGCCAAGGAGTTGACTGCCTTATTTGTCTTCCTAAGACCGCTTGGCT38340.11396780364958557No Hit
GTGAGCCAAGGAGTTGACTGCCTTATTTGTCTTCCTAAGACCGCTTGCCT36200.10760653344066243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT148100.087.809283
TAGGGTG182050.086.217854
ACCGACC11350.083.694363
AGGGTGT184500.083.682895
TGTAGGG136850.083.2028052
AACCGAC10950.081.9115142
GTGTAGG133150.081.1683041
CCACGGA21900.079.057546
CACGGAG25400.078.437137
GCCACGG18000.076.2502755
CGGAGTT25450.076.198049
ACCGATC6400.074.955483
AACGAGT19050.074.7784968
AAACCGA19200.074.709571
CCGACCC12100.074.136164
CAACGAG19800.073.4848257
ACGAGTT20500.072.6782469
CGACCCC11150.072.40865
GCCAACG16100.071.974735
ATGTTTG171200.071.4314655
Produced by FastQC (version 0.11.5)