FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241798
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTT61880.49830970898648574No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTT43410.3495737632046436No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT42100.33902454344426386No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT36330.29255965946152274No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT34360.2766955656233945No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT33000.26574370388742774No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT31420.2530202174588782No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT27770.2236273532410263No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT27220.21919829150956918No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT21690.1746660890096457No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT20320.16363369887856155No Hit
GAGAGGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT20040.1613789038152743No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT18650.15018545689395538No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT17040.1372203852800536No Hit
GAGAGGCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT16970.1366566865142318No Hit
GTGAGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCTC15920.12820120502690455No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT15250.12280580255403857No Hit
GAGGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT12960.10436480007215344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG254.8949187E-794.085285
AGTTGCG9200.086.422273
ACGGAGG9500.086.169283
GATGCGT13150.085.1418154
TGTTGCG33100.084.8541643
TCGGACG1450.084.359143
TGATGCG7600.082.950323
AGATGCG5950.082.2324453
GTTGCGT43500.082.189444
GACGCAA1300.079.617063
ATGCGTG13900.079.5325245
TGAGTTG54350.079.431681
CGGACGC2450.078.724424
TTGCGTG46250.077.3025055
TGCGTGT61900.075.626576
GCGTGTC5050.075.381417
GTGTAGG61850.075.0403751
TGTAGGG58200.074.317892
ACGGACG950.074.283873
GAGCCAA39100.073.9988863
Produced by FastQC (version 0.11.5)