FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630102
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT62960.38623349949880437No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT55380.33973334183995846No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT55060.3377702744981602No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTT52190.3201640142764072No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT50740.311268865383884No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT42980.26366448234527656No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT41120.25225415342107427No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT38590.23673365224998189No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTT36530.22409640623715568No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT33970.20839186750276975No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT31960.19606135076209955No Hit
GAGAGGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT29170.1789458573757961No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTT27970.1715843548440527No Hit
GAGAGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT26180.16060344690086878No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT25970.15931518395781366No Hit
GAGAGGCAAGCGGTCTTAGGAAGACAAATAAGGCAGTCAACTCCTTGGCT25340.1554503951286484No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT23050.14140219446390473No Hit
GTGAGCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCTC22650.1389483602866569No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTT21750.13342723338784934No Hit
GAGGGCCAAGCGGTCTTAGGAAGACAATGTCATAAATCAACTCCTTGGCT20620.12649515183712431No Hit
GAGAGGCAAGCGGTCTTAGGAAGACAATTAATTAAGGCAACTCCTTGGCT16930.10385853155201331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC201.834256E-594.0399255
CCGACGC201.834256E-594.0399254
TGTTGCG44750.087.8514563
TGATGCG9600.085.234173
AGTTGCG9450.085.0942153
GTTGCGT56100.083.898354
GATGCGT15900.082.506724
ACGGAGG14000.081.287373
AGATGCG6500.081.0289843
TCGGACG1850.078.7999043
TTGCGTG60650.077.836835
GAGCCAA57300.077.719693
TGAGTTG65850.077.4405751
TGCGTGT79200.076.132476
ATGCGTG17300.075.558085
AGCCAAG54800.075.506514
GGGCCAA30050.075.1160053
GTGTAGG79450.074.822861
GTGTTGC55650.074.654422
GGCAAGC50150.074.6318745
Produced by FastQC (version 0.11.5)