FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21418692
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG420650.084.442113
TGTGTTG6503650.080.638181
TGTCGGT92100.076.5181352
GTTGCGT477850.074.767534
CTGTTGG1390650.074.169471
GTGTCGG68200.072.080011
GTTCGGT104850.071.7002262
CTTGCGT57950.071.2177051
GCGTGTC57450.068.9228447
CGTTCGG8500.067.942061
TCGGTGT289200.067.5354
GTGTTGC575700.067.474282
CTGTCGG32550.062.890931
GAGTTGG331800.062.6839452
AGTTGGG299800.062.2500153
GTCGGTG107400.061.5926743
TCGGTCT45200.060.521764
TGTTCGG77700.060.366641
TGCGTGT616650.059.4422456
TTGCGTG615150.058.0158425
Produced by FastQC (version 0.11.5)