FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27078106
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTGG2408500.087.175231
TCTTGGG2737000.083.1774142
CGTTCGG112950.082.242551
CTTTCGG243800.082.015771
CTCTCGG283000.081.563581
GGTGTCG577850.080.6537558
CGGTGTC437300.076.8761145
CTTGCGT336000.074.3948061
CTATTGG356850.073.387441
TCGGTGT1997350.073.210854
GGTCTGT3082000.073.0062567
TATTGGG571850.072.3842852
TGTGTTG9755450.071.958691
CTCGGTG450000.071.2785643
GTGTCGC416850.070.99739
TCTCGGT402350.070.3779452
CGGTGTG1610300.069.694055
CTTGGGT3452700.069.602673
CCTTCGG73750.069.276111
GGTTCGG146300.069.2671051
Produced by FastQC (version 0.11.5)