FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11666814
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT82550.082.7814252
CTTGCGT59500.081.777911
TGTTGCG183750.081.365093
TGTCGGT59150.080.080652
TCGGTGT189750.079.413184
CGTTCGG6700.078.816381
TGTGTTG3150300.075.7681051
TGTTCGG61500.075.668531
AGTTGGG278600.075.6130753
GTGTCGG44700.075.4174351
GAGTTGG310750.075.014542
CTGTTGG560450.074.2085951
TCGGACG5800.072.2348863
TTCGGTG135500.071.532273
TGAGTTG346700.071.369591
AGTTGCG18500.070.738853
TTCGGTC20650.069.0728763
TCGGTCT29950.068.058094
CTGTCGG19850.067.457631
GGTTCGG25150.066.3649141
Produced by FastQC (version 0.11.5)