FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11666814
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGGG353800.085.861742
ATTGGGT851000.083.991443
TATTGGG487100.083.2272642
ATCGGTG110400.082.868483
GAATTGG343850.082.651931
GTATTGG423950.082.130361
TATCGGT65000.082.123962
CTATTGG179300.077.346861
CTATCGG25600.076.574831
AATCGGT37800.075.474522
CTCTTGG333950.075.408051
TAGGTGT700700.074.9712144
TCGGTGT728750.074.6768654
GTATCGG45050.073.953261
ATGAGTG357850.073.7812962
TCTTGGG521250.073.513572
CTTTCGG53300.073.4694061
CGGTGTG655000.073.083545
GGTTCGG78250.072.867481
ATAGGTG207600.072.5691763
Produced by FastQC (version 0.11.5)