FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18397500
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG343650.084.322343
GTTCGGT128600.083.261612
CTTGCGT79750.080.4703141
TGTGTTG5585450.079.638481
TGTCGGT102350.079.604112
TCGGTGT321300.078.878314
AGTTGGG520200.078.293713
GAGTTGG582100.078.2760542
CGTTCGG11900.078.059821
TGAGTTG639750.075.2382351
GTGTCGG75350.075.156751
TGTTCGG89500.074.601661
CTGTTGG954550.074.4527051
AGTTGCG30000.072.816143
CTGTCGG33750.070.414981
TTCGGTG227500.070.008623
GTCGGTG113250.069.75653
GTTGCGT441550.068.763264
GGTTCGG45650.067.7596741
TTCGGTC29750.067.731043
Produced by FastQC (version 0.11.5)