FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52299068
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG120550.012.460108145
TCGTGTG137500.07.3882923145
GTCGTGG128400.05.8774242145
CGGAAGG252100.05.8718643145
CGCGATC84500.05.83748053
TCTCGGG159350.05.4189305145
CGCCGTA96250.05.28753245-49
GCGCGAT85900.05.15236662
AGAGCGC156200.05.110111145
CCGACGA27055.2753858E-55.0968876145
AGCGTCG486650.05.0547733145
Produced by FastQC (version 0.11.5)