FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51414003
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG96500.012.859486145
TCGTGTG131800.06.8274903145
CGGAAGG244750.05.8708145
AGCGTCG346400.05.803055145
CGCCGTA70450.05.45946145
GTCGTGG120850.05.3443904145
AGAGCGG212050.05.201877145
CGACGAA23752.1690478E-45.194456145
GCGTCGT289450.05.099059140-144
CGTCGTG292350.05.063356140-144
ATCGGAG88950.05.058253145
Produced by FastQC (version 0.11.5)