FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55669644
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG124750.015.182346145
TCGTGTG140900.08.652411145
CGGAAGG264250.08.458136145
GAGCGTC651450.07.545079
CGCCGTA129350.07.45419645-49
ATCGGAG93650.06.973867145
AGAGCGC162350.06.8387713145
AGAGCGT724500.06.80389938
GTCGTGG124300.06.5969973145
TCGCCGT148600.06.381232345-49
GCCGTAT155650.06.325083345-49
AGAGCGG237500.06.141456145
CGTATCA169450.06.118008645-49
CCGTATC164950.06.109112345-49
TCTCGGG165850.06.081886145
GAGCGTG148100.05.8798347145
AAGAGCG883550.05.6201367
GTCGCCG177100.05.51811940-44
CGTCGGG115400.05.2192874145
CGGAAGA969450.05.2082194
Produced by FastQC (version 0.11.5)