FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63043674
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG122300.013.053919145
CGCCGTA93550.06.74196845-49
GAGCGTC520750.06.05585679
CGGAAGG296000.06.055499145
TCGTGTG153350.05.962542145
AGCGTCG456850.05.8136935145
TCGCCGT113800.05.64420445-49
AGAGCGT593500.05.43527468
CGTCGTG385150.05.369819145
GCCGTAT125000.05.3588745-49
CGTCGAG32502.7452552E-75.358862145
GCGTCGT378150.05.214226140-144
ATCGGAG104500.05.2082243145
CGTCGGG111900.05.058354145
GATCGGA795900.05.051216145
Produced by FastQC (version 0.11.5)