FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005753328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11113483
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG8456.531296E-610.298037145
GTCGTGT122750.06.9118485145
TAGATCG77500.06.736909145
CGCCGTA24702.9040166E-76.165272145
TCGTGTA118950.06.0962877145
AGCGTCG134500.06.038452145
CGTGTAG117400.05.9914727145
ATCGGAA203250.05.9225326145
AAGAGCG180850.05.8942523145
CGTCGTG123100.05.890767145
GCGTCGT125900.05.782208140-144
CGGTGGT57850.05.7661295145
GATCGGA204300.05.501653145
GAGCGTC149950.05.416284145
CGGAAGA197000.05.3742332145
GCCGTAT29851.6931808E-65.3445144145
TCGGAAG199150.05.3162136145
AGATCGG210850.05.185773140-144
AGAGCGG26604.041414E-55.1796675145
AGAGCGT160550.05.1490183145
Produced by FastQC (version 0.11.5)