FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005753355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46226654
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA213850.07.741966145
ACGAATC177450.06.956625145
ACACGTC476650.06.7031474145
AGTCACG252500.06.441875145
ATCGGAA740200.06.3275604145
CGTCTGA424100.06.249572145
AGATCGG794350.06.1338944145
GATCGGA756400.06.115241145
TCACGAA234450.06.008653145
CACGTCT508350.05.9423213145
CGAATCT178800.05.8887916145
TCTCGTA108300.05.833329145
TCGGAAG727200.05.711732145
ACGTCTG469800.05.703469145
TAGATCG295000.05.686115145
TCGTATG112950.05.657468145
CACGAAT225600.05.471866145
CGGAAGA719200.05.460913140-144
GCACACG602300.05.0998145
CACACGT594900.05.028969145
Produced by FastQC (version 0.11.5)