FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63134129
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG51550.010.405852145
AGCGTCG558500.06.632425145
CGTCGTG506350.06.5424433145
GAGCGTC637750.06.5129657145
CGTGTAG497800.06.3490124145
GTCGTGT516000.06.2515097145
TCGTGTA494350.06.1587048145
GCGTCGT518050.06.1258597140-144
GATCGGA870450.05.812811145
AGATCGG923650.05.7056036145
ACCGCGC172000.05.60724641
ATCGGAA863300.05.6006536145
AAGAGCG794650.05.591897145
TCGGAAG863900.05.4960723145
CGGAAGA867650.05.430545145
AGAGCGT703350.05.35928145
TAGATCG337650.05.3457317145
TGGTCGC158600.05.256168145
TCGGTGG251300.05.1633925145
CCGTATC144700.05.0597315145
Produced by FastQC (version 0.11.5)