FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63591706
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC152700.08.592573145
ACGTACG164150.08.258181145
GTACGCA175650.06.933377145
ACACGTC549650.06.6866007145
ACCGCGC162050.06.21464821
CACGTAC244950.06.0964003145
AGATCGG905950.06.0892544145
ACGTCTG555600.05.910438145
TACGCAA191900.05.892957145
ATCGGAA848800.05.824554145
AGTCACG300350.05.816652145
CGTCTGA487200.05.8028455145
CACGTCT601750.05.7703624145
TCACGTA286900.05.6850677145
GTCACGT285650.05.684568145
GCACACG710900.05.577794145
TCGGAAG842750.05.5395026145
CACACGT704900.05.501865145
GATCGGA854900.05.4862123145
CGGAAGA844150.05.4702034145
Produced by FastQC (version 0.11.5)