FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64331784
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG50600.012.892546145
CGTCGTG511500.07.014654145
AGCGTCG565250.06.745154145
TCGTGTA501650.06.617765145
CGTGTAG502750.06.314933145
GATCGGA889200.06.1952786145
GCGTCGT525300.06.145945140-144
GTCGTGT519600.06.068297145
GAGCGTC647450.06.0455275145
ATCGGAA882700.05.961701145
TCGGAAG874600.05.818008145
AGATCGG942200.05.6621504145
CGGAAGA872800.05.5642514145
AAGAGCG803850.05.4013023145
GTCGCCG132150.05.375336145
ACCGCGC179200.05.3290411
AGAGCGT713200.05.30524140-144
CAGATCG283200.05.2981443145
TAGATCG357400.05.171688145
TGGTCGC159400.05.1385055145
Produced by FastQC (version 0.11.5)