FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53732779
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC147850.011.3334071
GCGTCGG45050.011.261059145
CGCGATC90400.09.7281853
CTCACGC448150.09.649583
GCGCGAT89400.09.598592
GCTCACG450400.09.42925552
ACCGCAC154400.08.3926921
CACGCCT596250.07.80345
TCACGCC575150.07.7563714
CGCCTGT619250.07.56144487
ACGCCTG573850.07.5396786
GGCGCGA115850.07.4569511
CCGCGCC240350.07.23130562
GCGATCT186150.07.0920684
CGCGCCC201200.06.5383093
CGTCGTG398300.06.5322104145
AACGCGA29755.6024874E-106.5085721
ACCGTGC170850.06.40769241
AGCGTCG437850.06.2235546145
GGCTCAC1433950.06.07127331
Produced by FastQC (version 0.11.5)