FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70251404
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG55950.014.153882145
TCGTGTA921500.06.8907304145
CGCCGTA209900.06.65257145-49
AGCGTCG1054900.06.377476145
GAGCGTC1161650.06.3355355145
CGTGTAG903050.06.323535145
TAGATCG612600.06.2737594145
AGATCGG1660200.06.117809145
CGTCGTG959800.06.0783257145
ATCGGAA1580500.06.0309887145
GCCGTAT241050.05.99139445-49
TCGCCGT231550.05.91157145-49
GCGTCGT987050.05.8901076140-144
GTCGTGT954250.05.809136145
TCGGAAG1543300.05.7856307145
CCGTATC253000.05.754252445-49
CGTATCA260350.05.725471545-49
GATCGGA1613550.05.6778226145
GTCGCCG255050.05.638186540-44
AAGAGCG1376950.05.635108145
Produced by FastQC (version 0.11.5)