FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70634828
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG54600.013.684467145
CGCCGTA205850.06.86703545-49
AGCGTCG1080400.06.586698145
CGTGTAG918700.06.522133145
CGTCGTG979150.06.5121284145
GTCGTGT967750.06.2365365145
GAGCGTC1182200.06.19746145
TAGATCG636450.06.1547837145
TCGTGTA933550.06.1075683145
GCCGTAT237200.06.087796745-49
AGATCGG1706500.06.0744824145
TCGCCGT231500.05.93081645-49
AAGAGCG1409400.05.929274145
CCGTATC247950.05.882329545-49
TCGGAAG1588150.05.8739843145
AGAGCGT1278600.05.849346145
GCGTCGT1010950.05.8489776140-144
ATCGGAA1625900.05.7956033145
CGTATCA255300.05.792484345-49
GATCGGA1661950.05.726631145
Produced by FastQC (version 0.11.5)