FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69032891
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG194700.06.724051545-49
ACACGTC944050.06.511111145
CGTCTGA829600.06.4886794145
ATCGGAA1485550.06.2188454145
TAGATCG584550.06.1972785145
TCGGAAG1444450.06.0936317145
CACGTCT986650.06.053037145
GATCGGA1517600.06.0108175145
GCACACG1142750.05.9009485145
AGATCGG1556500.05.897984145
CGTATGC224500.05.837962645-49
GCCGTCT231100.05.809256650-54
CGGAAGA1421500.05.7621536145
ACGTCTG919950.05.724897145
CAGATCG688200.05.6972785145
ATGCCGT234400.05.67798545-49
TGCCGTC226050.05.6632550-54
CACACGT1121950.05.6472626145
CTCGTAT222250.05.63607840-44
CGCAATC341200.05.372604145