FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60341427
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC389850.07.216291145
ACGTACG409850.06.4749436145
CGTCTGA949000.06.2650924145
TCACGTA544750.05.962942145
GTCACGT550400.05.9544253145
GTACGCA398700.05.8558435145
ACGCAAA437500.05.7839856145
TACGCAA405900.05.7162437145
AGTCACG578050.05.707236145
CGCAAAT378450.05.690203145
CACACGT1235400.05.6754293145
CACGTAC493000.05.632896145
ACGTCTG1026900.05.6203675145
CACGTCT1095650.05.591968145
TAGATCG616950.05.5706806145
AGATCGG1682950.05.467266145
CGGAAGA1547150.05.4456983145
ATCGGAA1608300.05.427993145
CGTATGC261050.05.4161506145
GCACACG1259400.05.36577145
Produced by FastQC (version 0.11.5)