FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60414813
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG410550.06.9045463145
CGTACGC387600.06.6587186145
TACGCAA404000.06.4243045145
GTACGCA395800.06.3925495145
AGTCACG583700.06.3095512145
CGTCTGA956900.06.1595426145
TCACGTA551650.06.1241636145
CACGTAC498850.05.943986145
ACGTCTG1037000.05.858544145
TAGATCG628650.05.6508217145
GTCACGT556050.05.6324115145
ACACGTC1079150.05.623145
GCACACG1276450.05.4865303145
CACGTCT1103350.05.453675145
GATCGGA1649600.05.4276614145
AGATCGG1698200.05.4089403145
ATCGGAA1615150.05.368374145
CGTATGC257700.05.2607985145
TCTCGTA262600.05.1902413145
CGGAAGA1557400.05.176415145
Produced by FastQC (version 0.11.5)