FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64884150
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC172450.07.83303261
ACACGTC1125500.06.428531145
CTCACGC519650.06.4037123
CGTGATA376500.06.3351603145
CGCGATC105400.06.25937273
CACAACG515050.06.1230326145
CGTCTGA992500.06.121251145
ACGTCTG1086000.06.10159145
TATGCCG211750.06.03207845-49
GCTCACG536950.06.02186542
TCGGAAG1708750.05.8210626145
GCACACG1342200.05.8011456145
AGATCGG1845300.05.791048145
GCGCGAT105300.05.783372
CACGTCT1155900.05.6636205145
ACGTGAT449750.05.657996145
CAACGTG541450.05.570083145
TATCTCG282750.05.5639577145
CCGCGCC253450.05.5493162
CACACGT1307550.05.5445766145
Produced by FastQC (version 0.11.5)