FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64792173
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG75600.015.342236145
ACCGCGC177600.07.8925871
AGCGTCG1209500.06.095447145
GAGCGTC1313050.05.9294367145
CGCGATC107650.05.92632153
GCGCGAT107000.05.8948382
TCGTGTA1036550.05.8256607145
CGTCGTG1096350.05.792224145
CGTGTAG1031050.05.716119145
TAGATCG663150.05.575052145
AGATCGG1871300.05.547407145
GTCGTGT1084300.05.515628145
AAGAGCG1554050.05.4670506145
GCGTCGT1127700.05.464146140-144
CGCCGTA232000.05.38708445-49
GATCGGA1813600.05.376148145
GTCGCCG292150.05.3100467145
ATCGGAA1775200.05.2964287145
CTCACGC513100.05.25602633
CGGAAGA1722700.05.2516456145