FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56948053
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG70100.017.890545145
AGAGCGG179900.06.0847297145
CGTCGTG1298100.05.7632446145
AGCGTCG1424800.05.6984878145
CGTGTAG1211100.05.530794145
GTCGTGT1271850.05.4889073145
GTGGTCG428750.05.4274573145
TCGTGTA1215550.05.3793426145
TCGGTGG534050.05.3753753145
TGGTCGC398950.05.360423145
GTCGCCG358700.05.3152037145
TCGGAAG1982750.05.2758675145
GCGTCGT1340400.05.215729140-144
AAGAGCG1768550.05.209832145
AGATCGG2127400.05.1999707145
GTGTAGG1372550.05.1443145
GAGCGTC1524150.05.048282140-144
TAGATCG735250.05.02841145
CGCCGTA300250.05.0219903145
ATCGGAA2026100.05.0127125145
Produced by FastQC (version 0.11.5)