FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53249557
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91300.08.70286745-49
TCGGAAG502350.07.43217953
ATCGGAA502400.07.4045132
GATCGGA498550.07.39502331
CGTATGC109000.07.342858345-49
CGGAAGA511150.07.2612824
CTCGTAT103950.07.2388440-44
TCTCGTA110900.06.824409540-44
TGCCGTC120600.06.60012250-54
AGATCGG509950.06.439711145
GCCGTCT127200.06.405840450-54
ATGCCGT127400.06.32787545-49
TCGTATG122000.06.0374945-49
CGTCTGA305200.05.8193884145
ATCTCGT140800.05.51935440-44
TAGATCG224150.05.4980154145
CGTCTTC159000.05.37087650-54
AGAGCAC762000.05.18482458
CCGTCTT160000.05.1832650-54
ACACGTC340500.05.118212140-144
Produced by FastQC (version 0.11.5)