FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53249557
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC419300.08.8692839
AGAGCGT472450.07.99429758
GCGTCGG48250.07.062096145
CGCCGTA108500.06.89510945-49
AAGAGCG548650.06.88399847
ATCGGAA557150.06.77996162
TCGGAAG560350.06.66328953
GATCGGA554250.06.61009171
CGGAAGA578650.06.47736364
TCGCCGT121800.06.0588745-49
CGTCGTG331050.05.9129367145
GCCGTAT136050.05.8718445-49
AGATCGG569950.05.698681145
CCGTATC145950.05.54308145-49
TAGATCG250500.05.527881145
GTCGCCG141350.05.45684540-44
CGTATCA155050.05.36736745-49
TCGTGTA337750.05.2375426145
GCGTCGT337700.05.1737866140-144
AGCGTCG362400.05.0492134140-144