FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66399355
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG57500.018.034687145
CGTCGTG782250.06.61901145
CGTGTAG746700.06.4776893145
AGCGTCG861100.06.467673145
TCGTGTA754300.06.037483145
GAGCGTC946450.05.8844247145
GCGTCGT802000.05.874905140-144
GTCGTGT776900.05.766141140-144
GGTCGCC239700.05.566602145
GTCGCCG204300.05.537282145
AGATCGG1342800.05.5246496145
CGCCGTA160000.05.484106145
TCGGAAG1254850.05.4437666145
GTGTAGG938200.05.371923145
ATCGGAA1271650.05.3433347145
AGAGCGT1033200.05.223968140-144
GATCGGA1291550.05.204859145
CGGAAGA1250250.05.1853857145
AAGAGCG1140550.05.150046145
CTCGGTG376450.05.1433296145
Produced by FastQC (version 0.11.5)