FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67626807
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG55100.015.011398145
AGCGTCG882750.06.5260544145
CGTCGTG802950.06.5150037145
CGTGTAG769100.06.2640204145
TCGTGTA770150.06.001116145
GAGCGTC973800.05.9307723145
GCGTCGT828550.05.7607527140-144
TAGATCG516350.05.578459145
GTCGTGT793600.05.572323140-144
TCGGAAG1305100.05.5371137145
GATCGGA1345450.05.462731145
GTCGCCG209350.05.371885145
CGGAAGA1289600.05.344862145
AGATCGG1395450.05.334589145
ATCGGAA1324700.05.31826145
TGGTCGC240600.05.2471266145
AGAGCGT1060550.05.2210355140-144
AAGAGCG1181400.05.2018085145
TCGCCGT183600.05.1373434145
GTGTAGG968650.05.0859356145