FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56304933
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC97250.013.0556453
GCGCGAT97400.012.8910652
ACCGCGC162400.012.4164081
GCGTCGG50200.011.992796145
CTCACGC473350.010.6218643
GCTCACG479300.010.6148092
GGCGCGA122650.010.1859481
ACCGCAC160650.09.7775641
GCGATCT203700.09.0464934
ACGCCTG612000.08.4333696
ACCGTGC181500.08.3323341
CACGCCT641050.08.2661225
TCACGCC614150.08.1510244
CGCCTGT674250.08.06337
CCGCGCC260600.07.79803562
CGCGAGT36200.07.2151243
AACGCGA35000.07.09714751
CGCAATC101050.06.89262773
ACTGCGC167500.06.8915231
GGCTCAC1513000.06.88095761
Produced by FastQC (version 0.11.5)