FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57334481
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC95500.012.8335623
GCGCGAT96900.012.4255622
ACCGCGC163050.011.8636981
CTCACGC487450.011.7086963
GCTCACG491450.011.6890052
GGCGCGA120300.010.3152151
GCGTCGG46650.09.326868145
TCACGCC629250.09.1969774
GCGATCT202050.09.1167554
CACGCCT649800.09.058595
ACCGTGC180400.08.9018681
ACGCCTG626600.08.7575816
ACCGCAC167100.08.5183041
CGCCTGT680250.08.4718297
CCGCGCC268350.07.4329772
GGCTCAC1545800.07.39964151
CCTGTAA1837950.06.7680299
GCTCACA770900.06.62378642
CGATCTC340000.06.50287255
CCGTGCC224300.06.4997782
Produced by FastQC (version 0.11.5)