FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58134479
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC170000.012.6782341
GCTCACG504850.011.8123522
CTCACGC501950.011.6566553
CGCGATC101250.010.6696983
GCGCGAT101750.010.1959712
GCGTCGG49850.09.751222145
TCACGCC648350.09.1699134
ACCGTGC182750.08.99521
CACGCCT664950.08.9837115
ACGCCTG638150.08.6907296
GGCGCGA126050.08.5204031
CGCCTGT698550.08.3232787
GCGATCT212200.08.0294054
GGCGCAA120050.07.9725051
GCGCAAT106800.07.9477022
CCGCGCC278150.07.7725612
CGCAATC108750.07.66707043
ACCGCAC172350.07.54561041
ACGCGAG40250.07.3900092
GGCTCAC1602050.07.22834541
Produced by FastQC (version 0.11.5)