FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58030784
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC169500.012.25357151
CGCGATC98750.011.9745073
GCGCGAT98900.011.74257852
CTCACGC489900.011.3726313
GCTCACG493050.011.2324212
GCGTCGG51000.010.960151145
GGCGCGA128100.09.6144641
ACCGCAC168950.09.058341
GCGATCT206250.08.934024
CACGCCT656450.08.7129625
TCACGCC635850.08.7051654
ACGCCTG631900.08.6384756
CGCCTGT690750.08.4272357
CCGCGCC272150.07.97448442
ACCGTGC185750.07.9251971
ACGCGAG38850.07.66006762
GGCTCAC1576800.07.1915171
AACGCGA36000.07.0852151
ACTGCGC171050.07.02988771
CCTGTAA1886700.06.74318559
Produced by FastQC (version 0.11.5)