FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61678072
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG57650.010.061516145
ACCGCGC177200.09.923741
CGCGATC101150.09.2478463
GCGCGAT101350.09.2416542
GCTCACG516550.08.349452
CTCACGC512300.08.2944963
GGCGCGA131800.07.50492241
ACCGCAC176450.06.89309551
TCACGCC668200.06.44609554
CACGCCT692750.06.4459745
GCGATCT219650.06.40453054
AGCGTCG539500.06.397175145
CCGCGCC282950.06.36393552
ACGCCTG667050.06.25962166
CGCCTGT731000.06.12880567
CGCAATC107550.06.00062273
ACCGTGC196300.05.9721071
CGTCGTG487000.05.9701896145
ATCGGAA820000.05.8535166145
GTCGTGT499600.05.7905946145
Produced by FastQC (version 0.11.5)